What does peak mean in pharmacology


Synonyms: derivatisation
English: derivatization

1 definition

As Derivatization in chemistry the process for the formation of a derivative is called. This is the chemical change of a substance in order to change its chemical-physical properties (for example, to convert actually colorless substances into colored substances).

2 pharmacy

In pharmacy, derivatization is used to construct new, more effective or better tolerated drugs from existing drugs. For example, acetylsalicylic acid was created through derivatisation from salicylic acid.

3 chromatography

In chromatography, derivatization is carried out, for example, when the substances do not absorb light or do not fluoresce in UV light. In thin layer chromatography (TLC) they can then be made visible by applying detection reagents to form a colored derivative. This is called "post-chromatographic derivatization". Another example is gas chromatography (GC). Here, substances are derivatized, among other things, in order to be able to detect them more easily and more precisely.

3.1 Derivatization reactions

  • Thermochemical reactions and photochemical reactions: lead to the formation of a derivative by decomposition
  • Spraying detection reagents onto TLC plates
  • Dip from TLC plates
  • Evaporation of TLC plates with iodine / chlorine
reagent proof
NinhydrinAmino acids, amines
Vanillic sulfuric acidAlcohols, essential oil, steroids
Anisaldehyde-sulfuric acid Polyalcohols, steroids, terpenes
Potassium permanganate Polyalcohols, polycarboxylic acids, unsaturated compounds
Steaming with iodine multiple substance detection
Steam with chlorine Acid amides
PrimulinLipids, alkanes
2,2-diphenyl-1-picrylhydrazyl (DPPH) Antioxidants
3-dansylpropanamide (DPA) Sugar and sugar derivatives